HPLC Column, RP C4 Butyl, Wide Pore, 5um, 2.1mm ID x 50mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C4™ Wide Pore is a reversed-phase HPLC stationary phase designed for the separation of proteins, peptides, enzymes, and other large hydrophobic or neutral compounds. Manufactured using a four-carbon butyl ligand bonded to ultra-high purity silica and fully double end capped, this 300 Å wide pore stationary phase provides improved accessibility for large biomolecules while maintaining the reduced hydrophobic retention characteristic of C4 chemistry. The wide pore design makes it particularly well suited for bioanalytical applications where larger molecules require enhanced access to the bonded phase.
68504-05P-2-3M is an HPLC column packed with 5 µm RP C4™ Wide Pore particles featuring a 300 Å pore size in a 2.1 mm ID x 50 mm format. This short, narrow-bore configuration combines rapid analysis times, reduced solvent consumption, and compatibility with LC-MS instrumentation. The 5 µm particles provide low backpressure and robust performance, while the 300 Å pores improve mass transfer for larger biomolecules. Typical operating flow rates are approximately 0.2–0.3 mL/min. Standard HPLC systems used with 2.1 mm ID columns must be capable of precise and reproducible low-flow operation.
This column is particularly useful for bioanalysis involving proteins, peptides, enzymes, and other large biomolecules. The C4 bonded phase provides less hydrophobic retention than C8 or C18 phases, which can be advantageous when analyzing large molecules that would otherwise exhibit excessive retention. The 2.1 mm ID format is frequently used for LC-MS methods requiring high sensitivity and low solvent consumption.
Key Benefits
RP C4™ Wide Pore stationary phase
USP L26 equivalent chemistry
Butyl bonded phase
Fully double end capped
Ultra-high purity silica support
300 Å wide pore design
Optimized for proteins and peptides
Lower hydrophobic retention than C8 phases
5 µm particle size
Excellent for LC-MS bioanalysis
Typical Applications
Protein analysis
Peptide analysis
Enzyme analysis
Bioanalytical methods
LC-MS methods
Biomolecule characterization
Pharmaceutical analysis
Biopharmaceutical analysis
Reversed-phase HPLC
Method development
Specifications
Product Type: HPLC Column
Stationary Phase: RP C4™ Wide Pore
USP Classification: L26
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double End Capped
Particle Size: 5 µm
Pore Size: 300 Å
Column Dimensions: 2.1 mm ID x 50 mm
Length: 50 mm
Typical Flow Rate: 0.2–0.3 mL/min
Brand: Cogent
Important Notes
Designed for proteins, peptides, enzymes, and other large biomolecules
300 Å wide pores improve accessibility for larger analytes
Provides less retention than C8 and C18 stationary phases
Suitable for reversed-phase HPLC and LC-MS applications
The 2.1 mm ID format is ideal for reduced solvent consumption and MS compatibility
Standard HPLC systems must be capable of reliable operation at approximately 0.2–0.3 mL/min
Detector flow cells should be optimized for low-volume, narrow-bore chromatography
The 50 mm length is ideal for rapid analyses and high-throughput workflows
5 µm particles provide low backpressure and robust operation
Frequently used for bioanalytical and biopharmaceutical applications